קורס אתגרים באבחון במחלות מפגש 7 -16.02.2021
Body Fluid Culture
Aim of the test : Isolate and identify pathogenic organisms-
-Types of specimen
–Criteria of specimen rejection
Pleural Fluid: A collection of fluid in the plural space, normally found between the lung and the chest wal
lPeritoneal Fluid: A small amount of fluid in the peritoneal cavity that maintains moistness of the surface of the peritoneum
Pericardial Fluid:The area between the epicardium which is the membrane surrounding the heart muscle and the pericardium is called pericardial space and normally contain 15 to 20 ml of clear fluid.
Joint fluid:The fluid surrounding the joints and work as lubricant for joints.
Pre specimen processing
Who will collect the specimen
- Physician
Quantity of specimen
- 1-5 mL is adequate, a larger quantity of fluid is better.
Time relapse before processing the sample
- Body fluids should be treated as CSF specimens and should processed immediately.
Storage
Maintain specimen at room temperature. Do not refrigerate
Specimen processing: Body fluids for culture should be concentrated by either filtration or high speed centrifugation..
Infection of Sterile Body fluid,
Post specimen processing: Interfering factors, Result reporting, Turn around time
- CSF functions, Indications for analysis
:Cerebrospinal Fluid (CSF)
Meningeal infections, Subarachnoid hemorrhage, CNS malignancy
Specimen collection and handling
Specimen collection and handling
Tube 1 – chemistries and serology
Tube 2 – microbiology cultures
Tube 3 – hematology
Appearance
Cerebrospinal Fluid (CSF) – procedures
Differential Diagnosis of Meningitis
Cerebrospinal Fluid (CSF) – Molecular diagnosis
. Cerebrospinal Fluid (CSF)- microbiology by Laboratory Results
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