17.02.2021קורס ביוטכנולוגיה מפגש 14
The massively parallel sequencing technology known as next–generation sequencing (NGS) has revolutionized the biological sciences. With its ultra-high throughput, scalability, and speed, NGS enables researchers to perform a wide variety of applications and study biological systems at a level never before possible.
While the Sanger method only sequences a single DNA fragment at a time, NGS is massively parallel, sequencing millions of fragments simultaneously per run. … NGS also offers greater discovery power to detect novel or rare variants with deep sequencing..
WGS provides the most comprehensive data about a given organism. Using next generation sequencing can deliver large amounts of data in a short amount of time. Since you are profiling the entire genome, it allows for the discovery of previously unknown genes or variants.
The principle behind microarrays is that complementary sequences will bind to each other. The unknown DNA molecules are cut into fragments by restriction endonucleases; fluorescent markers are attached to these DNA fragments. These are then allowed to react with probes of the DNA chip.
The overall function of alternative splicing is to increase the diversity of the mRNA expressed from the genome. Due to the combinatorial control mechanisms that regulate alternative exon recognition, splicing programs coordinate the generation of mRNA isoforms from multiple genes.
Fluorescence in situ hybridization (FISH) is a laboratory technique for detecting and locating a specific DNA sequence on a chromosome. The technique relies on exposing chromosomes to a small DNA sequence called a probe that has a fluorescent molecule attached to it.
Pre –implantation genetic screening
PGS (also known as aneuploidy screening) is a treatment add-on. It involves checking the chromosomes of embryos created by in vitro fertilisation (IVF) or intracytoplasmic sperm injection (ICSI) for abnormalities. … During PGS, a single cell or a small number of cells is removed from the embryo.