ביוטכנולוגיה

סיכום שיעור 7

hanna morani

hanna morani

פרטי הסיכום

קורס: ביוטכנולוגיה

מספר השיעור: 7

סיכום השיעור

Major Advances in Macromolecular Crystallography
– Robots and high-throughput screening methods
for crystallization
– Brilliant, tunable X-rays from synchrotron
radiation facilities
– Methods for phase determination based on multiple
wavelength anomalous diffraction
– Enhanced high-power computers with excellent
software
– Cryo-Crystallography

Seven basic steps to determine protein crystal structure
1. Obtain homogeneous sample preparation
2. Grow suitable crystals
3. Collect intensity data
4. Calculate an electron density map
5. Interpret the map by building an atomic model
6. Refine the model

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